BAX (Bcl-2 Associated X protein) ELISA Assay

The protein encoded by BAX gene is related to Bcl-2 protein family, which act as  an anti- or pro-apoptotic regulators. This protein interacts with, and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the lack in membrane potential and the release of cytochrome c.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat BAX. After adding samples/standards, a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of BAX conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat BAX.