CASP-3 (Caspase-3) ELISA Assay

Caspase 3 is a comparatively small protein that composed of 2 subunits, a 12- and 17-kDa subunit. It plays a critical role in programmed cell death (apoptosis) and catalyzes the specific cleavage of many key cellular proteins. It’s also significant for normal brain development.

Assay Principle

ELISA plate is pre-coated with an antibody specific to Rat CASP3. After adding samples/standards, a biotinylated detection antibody specific for Rat CASP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of CASP-3 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value measured is proportional to the concentration of Rat CASP-3.