COX 1 & COX 2

PTGS-1/COX-1 (Prostaglandin Endoperoxide Synthase 1 / Cyclooxygenase-1) ELISA Assay

COX-1 is a complete membrane protein and it is located in the endoplasmic reticulum. It is a
bi-functional protein. COX-1 is the responsible enzyme to synthase PGE1 and PGI1 that exert cytoprotective effects on many aspects of gastric functions. COX-1 is considered as a housekeeping enzyme, being responsible for cell-to-cell signaling and tissue homeostasis.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat PTGS1/COX-1. After adding samples/standards, a biotinylated detection antibody specific for Rat PTGS1/COX-1 and
Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of PTGS1/COX-1 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat PTGS1/COX-1.

PTGS-2/COX-2 (Prostaglandin Endoperoxide Synthase 2 / Cyclooxygenase-2) ELISA Assay

Prostaglandins are composed from arachidonic acid through the action of cyclooxygenase (COX). There are two closely forms of COX that are known as COX-1 and COX-2. COX-2 is upregulated during cancer and inflammation. Both contribute to auto-regulatory and homeostatic prostanoids generation.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat PTGS2/COX-2. After adding samples/standards, a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of PTGS2/COX-2 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat PTGS2/COX-2.