MMP-9 (Matrix Metalloproteinase 9) ELISA Assay

Matrix metalloproteinase (MMP)-9, one of the most widely investigated MMPs, regulates pathological re-modelling processes that involve inflammation and fibrosis in cardiovascular diseases. MMP-9 directly degrades extracellular matrix proteins and activates cytokines and chemokines to regulate tissue re-modelling.

Assay Principle

An ELISA plate is pre-coated with an antibody specific to Rat MMP-9. After adding samples/standards, a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of MMP-9 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MMP-9.