Cellular Drug Uptake By Flow Cytometry
- Cellular uptake plays an essential role in drug bioactivity, for example, the internalization and accumulation of drugs into cancer cells are crucial for the therapeutic effect against tumors.
- Flow cytometry has the advantage of measuring the fluorescence of individual Cells, accordingly it can measure the cellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
- Flow cytometry can measure all aspects of cellular pharmacokinetics including the intracellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
- with relatively weak fluorescence due to high laser excitation and a high-gain photomultiplier system.
- Non-fluorescent drugs can be evaluated as well after conjugation with a suitable fluorophore.
- Assessment of the cellular pharmacokinetics of fluorescent or fluorescent labelled drugs either concentration-dependent or time-dependent.
- Cellular Pharmacokinetic studies of formulated (nanoparticles) versus non-formulated drugs.
- Cellular pharmacokinetics studies of different drugs alone and in combination with cellular entrapment enhancer (Such as P-glycoprotein inhibitors) in naïve as well as efflux pump over expressing cells (such as MCF-ADR).
Types of Tests
- Concentration dependent
- Time dependent
- Formulated / non-formulated
- With entrapment enhancer