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Home All Services Tests Cell-Based Assays Cytotoxicity assays
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Cytotoxicity assays

Rated 4.65 out of 5 based on 26 customer ratings
(26 customer reviews)

$20.00 – $150.00 / compound

Cytotoxicity assays are widely used in the domain of pharmaceutical research. Applications include screening for cytostatic and cytotoxic anti-cancer drugs, measurement of cell proliferation (IC50 values) in response to different drugs, extracts, formulations, growth factors, cytokines, mitogens and nutrients, and assessment of physiological mediators that inhibit cell growth.

You can also check our other services:

1- Flow cytometric assays
2- Multipurpose treated-flasks
3- Cellular drug uptake by HPLC
4- Cell migration / Wound healing assay
5- Western Blotting
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SKU: N/A Categories: Cell-Based Assays, Tests Tags: antiproliferative, In-vitro Cytotoxicity, SRB
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  • Description
Description

Cytotoxicity assays

Cytotoxicity assays are widely used in the domain of pharmaceutical research. Applications include screening for cytostatic and cytotoxic anti-cancer drugs, measurement of cell proliferation (IC50 values) in response to different drugs, extracts, formulations, growth factors, cytokines, mitogens and nutrients, and assessment of physiological mediators that inhibit cell growth.

Determining the half-maximal inhibitory concentration (IC50) is a common objective of these assays. IC50 is a measure of the effectiveness of a substance in inhibiting a specific biological or biochemical function.

The assays can be conducted on different levels:

  1. Quick screening of cytotoxic activity: checking viability % of 2 concentrations of the test compound.
  2. Routine determination of IC50 (using a logarithmic serial dilution): testing 5 concentrations of the test compound.
  3. Thorough determination of IC50 (using logarithmic & half-log serial dilution): testing 10 concentrations of the test compound.

Available testing protocols

A. Sulforhodamine B (SRB) cytotoxicity assay

Sulforhodamine B (SRB) cell cytotoxicity assay is one of the most widely used methods to detect cell viability or drug cytotoxicity. It is simple, accurate, reproducible and sensitive. It is the method of choice for high cost-effective screenings. This method relies on the property of SRB (a bright-pink aminoxanthene dye), which binds stoichiometrically to proteins under slightly acidic conditions and then can be extracted using basic conditions; thus, the amount of bound dye can be used as a proxy for cell mass, which can then be extrapolated to measure cell proliferation. It can be measured at 565 nm.

This assay is independent of cell metabolic activity and therefore showing less interference by the testing compounds and is one of the most reproducible cytotoxicity assays ever.

B. WST-1 cell proliferation assay

WST-1 assay is an upgrade for MTT and MTS assays, which are colorimetric assays for measuring the activity of enzymes that reduce MTT or structurally related dyes (XTT, MTS, and WSTs) to formazan dyes. The assay is highly convenient as it requires neither washing nor harvesting or solubilization of cells. WST -1 yields water-soluble cleavage products. The generation of the dark yellow colored formazan is measured at 420-480nm (optimal at 440nm) and is directly correlated to cell number.

WST-1 is more stable, has a wider liner range and shows accelerated color development compared to other structures related dyes.

You can also check our other services:

1- Flow cytometric assays
2- Multipurpose treated-flasks
3- Cellular drug uptake by HPLC
4- Cell migration / Wound healing assay
5- Western Blotting

26 reviews for Cytotoxicity assays

  1. Shereen shaker – November 29, 2018

    Rated 5 out of 5

    Still

  2. ahmed salah (verified owner) – October 3, 2019

    Rated 5 out of 5

    I hope you add a comment about the result

  3. Eman Abd AL Mageed (verified owner) – October 7, 2019

    Rated 5 out of 5
  4. Eman Abd AL Mageed (verified owner) – November 6, 2019

    Rated 5 out of 5
  5. Mai A. (verified owner) – November 10, 2019

    Rated 4 out of 5

    The only thing that I was not keen of is the delay and not sticking to the time plan agreed upon. Other than that, everything was great.

  6. Abeer fouad (verified owner) – January 13, 2020

    Rated 5 out of 5

    Thanks for your help and a fast analysis and a good way by Dr Eslam

  7. kamel elnahal (verified owner) – February 10, 2020

    Rated 4 out of 5
  8. Mohamed Raafat (verified owner) – February 19, 2020

    Rated 5 out of 5
  9. Mostafa Baky (verified owner) – February 26, 2020

    Rated 5 out of 5
  10. Shaymaa Kassab (verified owner) – March 28, 2020

    Rated 5 out of 5

    Results are evidence-based by real photos. The team in charge is competent and very helpful. Above all of this flexible in handling sudden challenges.

  11. omnia eid (verified owner) – March 29, 2020

    Rated 5 out of 5
  12. reham gamal (verified owner) – May 8, 2020

    Rated 5 out of 5

    Thank you

  13. Shaymaa Kassab (verified owner) – May 11, 2020

    Rated 5 out of 5
  14. Sherif Fahmy (verified owner) – July 9, 2020

    Rated 5 out of 5
  15. Abeer Bayoume (verified owner) – July 29, 2020

    Rated 5 out of 5

    Very happy

  16. dalia elmasry (verified owner) – September 3, 2020

    Rated 3 out of 5
  17. Sherif Fahmy (verified owner) – September 5, 2020

    Rated 5 out of 5
  18. Sherif Fahmy (verified owner) – September 24, 2020

    Rated 5 out of 5

    I appreciate the support I received from Dr. Omar Sakr, Dr. Nancy and Dr. Mai.
    Excellent service and accurate results.

  19. Anonymous (verified owner) – October 6, 2020

    Rated 5 out of 5
  20. Yasmine Ramadan (verified owner) – October 22, 2020

    Rated 5 out of 5

    Excellent lab and I felt completely satisfied in dealing with it

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