HPLC-QTOF-HRMS

The application of mass spectrometry (MS) coupled to liquid chromatography (LC) has been recognized as a revolutionary breakthrough in the analysis and characterization of compounds. LC can efficiently separate compounds from a variety of complex samples, and MS, especially multistage (tandem), can be used to detect analytes with superior sensitivity. While DAD and fluorometric detectors can accomplish the same task with good sensitivity, insufficient selectivity and interference from molecules with similar chromatographic behavior remain the major drawback of these techniques.

The applications of LCMS in the area of natural products, as an example, is incomparable to other techniques including; preliminary screening and dereplication, quality control and fingerprinting, and metabolism and pharmacokinetics studies.

Electron spray ionization (ESI), and atmospheric pressure chemical ionization (APCI) are the most commonly used ionization techniques in such analyses. Despite their character as soft ionization techniques, which results in very little molecular fragmentation, the use of tandem mas provides supplementary information of fragments dissociated from molecular ion peak.

Examples of applications

• Dereplication of natural products
• Screening for unknown compounds in complex samples
• Pharmacokinetic studies of natural and synthetic molecules
• Detection of extrinsic materials in natural products and synthetic drugs (e.g. synthetic, pesticides, NDMA…etc)
• Identification of pure synthetic or natural compounds using HRMS

Instrument technical specs

• Device model: X500R LC-QTO, SCIEX, USA
• Mass Range TOF: Up to 40 kDa. Precursor ion selection: 5-2250 m/z.
• Mass Accuracy Over Time: Less than 2 ppm RMS over 12 hours of LC-MS
• Ionization Sources: Turbo V ion source with Twin Sprayer ESI Probe and Twin Sprayer APCI Probe
• TOF-MS Resolution and Speed: ≥ 42,000 (FWHM) measured on the (M+6H)6+ charge isotope cluster for bovine insulin at m/z 956

Sample condition

Samples should be provided in sufficient amounts (30 mg, or more), and the suitable solvent (for dissolving samples) should be stated.

Outcome to expect

1. Raw data (the file extension is convertible by MS converter to fit with your preferred software). Printable data can be provided upon request.
2. Data includes TIC in both negative and positive ion modes, in addition to MS2 for major peaks
3. The provided data do not include identification of the detected peaks. Identification is a scientific exercise that needs further spectral data to ensure accurate identification. This can be offered at extra fees.

Service Pricing

Price might vary based on sample nature, needed preparation, requested number of runs as well as report interpretation.