Alpha Amylase Inhibition Assay 

a-Amylase is a key enzyme in carbohydrate metabolism. It catalyzes the hydrolysis of alpha 1,4- glucan linkage in starch and related polysaccharides to produce oligosaccharides.  a-Amylase is normally produced and stored in salivary glands and pancreas and secreted into the digestive tract. Alpha amylase inhibitors help in the prevention and treatment of metabolic syndromes such as diabetes and obesity.

Principle of the Assay:

The assay is carried out using porcine alpha amylase and 2-chloro-4-nitrophenyl-α-D-maltotrioside as a chromogenic substrate. Upon hydrolysis by the enzyme the substrate turns to yellow colored product which can be measured colorimetrically and correlated to enzyme activity.

Assays available and the expected output:

1. Quick Screening

2. IC50 Determination

N.B: Kinetic measurement is not available right now

You can also check our other services:

1- Choline esterase inhibition assay

2- Alpha Glucosidase inhibition assay 

3- Pancreatic Lipase inhibition assay

4- COX inhibition assay