MicroRNA extraction & quantification
MicroRNAs are small non-coding RNA molecules that function broadly as negative regulators of gene expression to control a wide range of cellular processes. The study of microRNA is growing rapidly as researchers uncover the importance of these small regulatory elements linked to a wide range of biological functions.
Several cancers and diseases are associated with different profiles of microRNA expression.
microRNA extraction and determination of its expression determination could be done by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).
Two common methods for the detection of PCR products qPCR are:
(1) non-specific fluorescent dyes that intercalate with any double-stranded DNA
(2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence.
What exactly do we provide?
- MicroRNA extraction.
- Qualitative and/or quantitative detection of microRNA.
What are the sample acceptance/rejection criteria?
Samples can be tissues or cell culture pellets.
Outputs/deliverable: Quantitative reverse transcription PCR (RT-qPCR):
qPCR amplification curves, cycle threshold (Ct) values and relative abundance.