Quantitative viral load assay
Study of viral replication for RNA and DNA viruses is a hot topic in both research and diagnostics. physicians and scientists can quantitatively measure viral load and use it as a probe for testing the effectiveness of novel antiviral compounds, accurate diagnosis, or prediction of disease outcome.
Precision techniques for viral load quantification and taxonomic identification for research or clinical diagnostic applications include targeting both conserved and unique genomic loci and RT-qPCR amplification for RNA viruses or qPCR amplification for DNA viruses. Detection pipeline is done through different techniques ending with the full Sanger DNA sequencing.
What exactly do we provide?
- DNA or RNA extraction
- Identification of viral taxa
- Primer design and RT-qPCR for RNA samples
- Primer design and qPCR for DNA samples
- Quantitative PCR (qPCR) for virus load quantification
- Sanger sequencing
What are the sample acceptance/rejection criteria?
- Accepted samples include tissue samples, blood, infected cell pellets, swabs, extracted DNA and/or DNA, etc.
- Important note: sample collection and storage techniques have a great impact on the integrity of nucleic acid in your samples and are the sole responsibility of the researcher. Samples for RNA-based assays will be only accepted if have been stored in RNA later solution or at -80°C or in liquid nitrogen as applicable. If samples have been stored properly, RNA later or dry ice may be provided by Nawah sample transport upon the researcher’s request at an additional cost.
Outputs / deliverables:
- Quantitative PCR (qPCR): absolute quantity of target nucleic acid in the sample, qPCR amplification curves and cycle threshold (Ct) values
- Multiplex PCR: Identified viral taxa depending on the test kit and primers used
- DNA sequencing: Raw sequence data, chromatogram file, analyzed and annotated sequence data, virus identification.