PuriFlash 4100

Tuesday, 17 March 2015
PuriFlash 4100

Chromatographic separation has never been this fast!

Still using gravity-based columns? Get introduced to flash chromatography

Flash Chromatography is a rapid, simple and economical alternative to simple gravity-based column chromatography. Hereby, mixtures are separated via optimized pre-packed columns, through which mobile phase is pumped at a high flow rate. Higher flow rates can only be achieved via specially designed equipment that can handle high pressures, and this is when “Ultra Performance Flash Purification” could be achieved.
PuriFlash 4100 is a premium, easy to use Flash-Prep-LC system. With a flow rate up to 250 mL/min and a maximum pressure of 100 bars, It fully adapts the Ultra Performance Flash Purification concept to achieve accelerated and more efficient separations.

Higher purification efficiency reducing number of re-runs

The use of high pressure together with adapted columns with small particle spherical silica allows for higher loading, faster throughput, and reduced solvent consumption. Moreover, the computerized control of solvent mixing and gradient schemes allow you to get unprecedented resolution and separation efficiency. All together would cut the number of needed re-runs to a minimum compared to traditional gravity-based chromatography.

No need to waste weeks on work that can be done in days

Sample can be loaded via different ways (dry load and liquid injection) matching the nature of your mixture. Separation can be done over a wide range of column sizes and nature (RP and NP). Fractions are automatically detected using a wide range PDA detector, received in fraction collector, and finally stored for you in screw-capped tubes.

2 in 1 device: Switch between Flash and HPLC Prep mode to match your needs

PuriFlash 4100 combines the power of two important techniques: (1) Use Flash mode to treat large amounts of primary samples containing lots of compounds, to get fractions of improved purity.  (2) Use the HPLC-Prep mode to treat your secondary fractions and purify target compounds to the optimum possible purity.


- Pump: Flow rate: 250 mL/min - Pressure: 100 bars max - Gradient: Quaternary 
- Detector: PDA-UV-Vis 190 - 840 nm
- Fraction collector: 4 Racks, 176 tubes, 18 x 150mm
- Auto- Injector Valve module: including 6-ways electric valve w/loop module (liquid or Dry load injection) 
- Column Compatibility: Normal & Reverse Phase
- Sample size: Flash columns: 20 mg – 50 g, Prep HPLC columns: 20 mg – 100 mg


- Fast and efficient fractionation of crude extracts
- Purification of organic compounds for drug discovery research, medicinal chemistry, and pharmaceutical sciences.
- Separation of compounds up to gram quantities.
- Useful for research in agrochemicals, petrochemicals, pharmaceutical sciences, natural products, polymers, and catalysts.

Service prices

You have to choose one of two choices:

1. Rent the device and purify your samples yourself

Don't worry, we will train you!

- You will be asked to attend one of our “PuriFlash introductory courses”. Attendance is mandatory before you can start working. The course is offered several times per month. Many important technical and logistic questions will be answered during the course. Click here to visit the course page and register.
-Then, you can book the time slot that suits you. During your presence, a member of Nawah team will be there to offer technical assistance.

2. We can purify them for you

Our team members have extended experience in operating the device and purifying samples for clients since 2015. 

In both options: pay only what you actually used

With our new pricing system, you will pay a basic fee plus consumables you actually used. You are free to bring your solvents and consumables if you like to do so.

Price table:

NP: Normal phase, RP: Reveresed phase, Prep: Preparative, 

  Purify it yourself We purify it for you*
Basic Fee 200 EGP/4h slot 300 EGP/sample
NP Flash column (25g) 185 EGP (disposable column) 200 EGP (disposable column)
NP Flash column (80g) 365 EGP (disposable column) 400 EGP (disposable column)
NP Prep column 115 EGP/run 115 EGP/run
RP Flash column 70 EGP/run 70 EGP/run
RP Prep column 100 EGP/run 100 EGP/run
Solvents (Price/L)    
DCM HPLC grade 290 290
Methanol HPLC grade 95 95
Hexane HPLC grade 290 290
Ethyl acetate HPLC 395 395
Water HPLC grade 15 15
Acetonitrile HPLC grade 290 290
Ethanol HPLC grade 236 236
Isoprpanol HPLC grade 221 221
DCM Lab grade 86 86
Methanol Lab grade 52 52
Hexane Lab grade 105 105
Ethyl acetate Lab grade 112 112
Acetonitrile Lab grade 185 185
Ethanol Lab grade 80 80
Isoprpanol Lab grade 65 65

 * You will be charged according to actual solvent consumption, based on a"solvent consumption report" generated automatically by the device.  

Things to know before you send your samples

1. Check the solubility of your sample: You will be asked to state the solvent (or) mixture of solvents that can efficiently dissolve your sample and the volume needed to dissolve the supplied amount. (If your sample didn't dissolve in the stated solvent, we will have to evaporate the solvent using the Rotavap and charge you 50 LE for this step).
2. Check the weight of your sample. Minimum acceptable weight is 20 mg of dry weight. 
3. Check if your sample is heat or light sensitive. Upon submission and evaluation of your sample, our team will inform you if your sample would need any step that involves heating (e.g. dry loading).
4. Check UV activity: is your target compound(s) UV active?
5. Check the best mobile phase (MP) to separate your sample. You will be asked to send a TLC showing good separation of your compounds, and to state in details of the used MP. A Confirmatory TLC will be done by our team before we start.
6. You will pay per run of the device. Each run improves the purity of your target compound. We will contact you after each run to discuss results and plan the next step with you.
7. Separated fractions will be collected in glass tubes and stored in the fridge till you come and collect them.  Five days storage for free, extra charges for more days. Special screw capped tubes can be used upon your request for extra fees.
8. If you are bringing your own solvents, kindly note that only high grade solvents can be used.
9. Before your first time at Nawah, it’s recommended to talk to a member of the team to go through all steps and what you need to bring before you come. This will allow you to make the best use of your time at Nawah.
10. You will only pay the device rent and chemicals/consumables you purchase (columns, cartridges,...).  - Other services like drying on rotavap are available for extra charges.

Puriflash saves time and money by applying chromatographic conditions to your sample in an accelerated way.


if these conditions were not correct, we cannot guarantee an efficient separation. Take some time to develop and validate a method or let our team work it out for you (extra fees service).


How to send samples!

​External Resources