VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Assay

It’s a significant neuroprotectant in the central nervous system (CNS). VEGF-A signalling has a critical role in embryonic development through the regulation of angiogenesis. It regulates many of the endothelial response such as migration and proliferation of endothelial cells, vascular permeability. Angiogenesis is important for tumour progression and this process is highly dependent on VEGF-A signalling.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat VEGF-A. After adding samples/standards, a biotinylated detection antibody specific for Rat VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of VEGF-A conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat VEGF-A.