Biomarkers
MMP-9 (Matrix Metalloproteinase 9) ELISA Assay
EGP0.00 / SampleMatrix metalloproteinase (MMP)-9, one of the most widely investigated MMPs, regulates pathological re-modelling processes that involve inflammation and fibrosis in cardiovascular diseases. MMP-9 directly degrades extracellular matrix proteins and activates cytokines and chemokines to regulate tissue re-modelling.
Assay Principle
An ELISA plate is pre-coated with an antibody specific to Rat MMP-9. After adding samples/standards, a biotinylated detection antibody specific for Rat MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of MMP-9 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat MMP-9.
NFKB-p105 (Nuclear factor NF-kappa-B p105 subunit) ELISA Assay
EGP0.00 / SampleIt’s a transcription factor that exists in almost all cell types. It’s considered as the endpoint in many biological processes such as cell growth, differentiation, inflammation, apoptosis and tumorigenesis. It plays an important role in regulation process of inflammatory responses and also mediate the induction process of many pro-inflammatory genes in innate immune system. Unfortunately inappropriate activation of NFKB due to inflammatory diseases while continual inhibition of NFKB could cause improper immune cell development or delayed cell growth.
Assay Principle
ELISA plates are pre-coated with an antibody specific to Rat NFKB-p105. After adding samples/standards, a biotinylated detection antibody specific for Rat NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of NFKB-p105 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat NFKB-p105.
Oxidative Markers
EGP140.00 – EGP200.00 / sampleOxidative stress is injurious to cells causing damage to DNA, proteins and lipids. It is caused by increased levels of reactive oxygen species (ROS) or/and decreased levels of antioxidants including reduced glutathione (GSH). Oxidative stress has been implicated to play a role in ageing and many pathological diseases, including cancer, diabetes, cardiovascular disorders, atherosclerosis, Parkinson’s and Alzheimer’s Diseases.
Several assays were developed for the assessment of total antioxidant capacity as well as for specific markers.
Renal Function Tests
EGP80.00 / sampleThe kidneys play a vital role in the excretion of waste products and toxins such as urea, creatinine and uric acid, regulation of extracellular fluid volume, serum osmolality and electrolyte concentrations, as well as the production of hormones like erythropoietin, vitamin D and renin. Assessment of renal function is important in the management of patients with kidney disease or pathologies affecting renal function. Tests of renal function have utility in identifying the presence of renal disease, monitoring the response of kidneys to treatment, and determining the progression of renal disease.
TNF-α (Tumor Necrosis Factor Alpha) ELISA Assay
EGP0.00 / SampleTNF-α (Tumor Necrosis Factor Alpha) ELISA Assay
Tumor necrosis factor (TNF), a 17 kDa protein composed of 157 amino acids. It is mainly produced by activated T lymphocytes, macrophages, and natural killer (NK) cells. It plays a critical role in immune system for cell signaling. If macrophages detect any infection, immediately TNF is released to alert other immune system cells as part of an inflammatory response.
Assay Principle
The micro ELISA plate is pre-coated with an antibody specific to Rat TNF-α. After adding samples/standards, a biotinylated detection antibody specific for Rat TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of TNF-α conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat TNF- α.