8-OHdG (8-hydroxy-2′-deoxyguanosine) ELISA Assay

It’s a widely-used biomarker of oxidative DNA damages. 8-OHdG in peripheral blood leukocyte is associated with mutation and cancer risk. The level of 8-OHdG in peripheral blood leukocytes can indicate a long-term response to oxidative stress rather than that in urine. Accurate identification and quantification of leukocyte 8-OHdG are significant for understanding its mechanism of repair, formation, and biological consequences.

Assay Principle

ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate is pre-coated with 8-OHdG. During the reaction, 8-OHdG in the sample/standard competes with a fixed amount of 8-OHdG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 8-OHdG. Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The optical density is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat 8-OHdG.