Bcl-2 (B-cell Lymphoma/Leukemia 2) ELISA Assay

It’s encoded by BCL2 gene. Actually it was the first apoptosis regulator identified in organisms. Bcl-2 proteins family has a significant role in normal cell physiology related to neuronal activity, autophagy, calcium handling and mitochondrial dynamics as it exists to the outer membrane of mitochondria, so it promotes cellular survival and inhibits the actions of pro-apoptotic proteins.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat Bcl-2. After adding samples/standards, a biotinylated detection antibody specific for Rat Bcl-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of Bcl-2 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat Bcl-2.