Number of samples |
15 or less samples , More than 15 samples |
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![CASP-3-ELISA-Nawah](https://nawah-scientific.com/wp-content/uploads/2021/06/IMG-20221023-WA0022-300x300.jpg 300w, https://nawah-scientific.com/wp-content/uploads/2021/06/IMG-20221023-WA0022-150x150.jpg 150w, https://nawah-scientific.com/wp-content/uploads/2021/06/IMG-20221023-WA0022-600x600.jpg 600w)
![](https://nawah-scientific.com/wp-content/uploads/2021/05/Franz-Cell-no-label_small-300x300.jpg 300w, https://nawah-scientific.com/wp-content/uploads/2021/05/Franz-Cell-no-label_small-150x150.jpg 150w, https://nawah-scientific.com/wp-content/uploads/2021/05/Franz-Cell-no-label_small.jpg 400w)
BAX (Bcl-2 Associated X protein) ELISA Assay
EGP0.00 / Sample
The protein encoded by BAX gene is related to Bcl-2 protein family, which act as an anti- or pro-apoptotic regulators. This protein interacts with, and increases the opening of the mitochondrial voltage-dependent anion channel (VDAC), which leads to the lack in membrane potential and the release of cytochrome c.
Assay Principle
ELISA plates are pre-coated with an antibody specific to Rat BAX. After adding samples/standards, a biotinylated detection antibody specific for Rat IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of BAX conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat BAX.
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