It’s a transcription factor that exists in almost all cell types. It’s considered as the endpoint in many biological processes such as cell growth, differentiation, inflammation, apoptosis and tumorigenesis. It plays an important role in regulation process of inflammatory responses and also mediate the induction process of many pro-inflammatory genes in innate immune system. Unfortunately inappropriate activation of NFKB due to inflammatory diseases while continual inhibition of NFKB could cause improper immune cell development or delayed cell growth.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat NFKB-p105. After adding samples/standards, a biotinylated detection antibody specific for Rat NFKB-p105 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of NFKB-p105 conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat NFKB-p105.