Enzyme Inhibitory Assays
Alpha amylase inhibition assay
EGP300.00 – EGP600.00 / SampleAlpha Amylase Inhibition Assay
a-Amylase is a key enzyme in carbohydrate metabolism. It catalyzes the hydrolysis of alpha 1,4- glucan linkage in starch and related polysaccharides to produce oligosaccharides. a-Amylase is normally produced and stored in salivary glands and pancreas and secreted into the digestive tract. Alpha amylase inhibitors help in the prevention and treatment of metabolic syndromes such as diabetes and obesity.
Alpha glucosidase inhibition assay
EGP250.00 – EGP500.00 / SampleAlpha-glucosidase stands as a target for the treatment of several diseases. It is a key enzyme in carbohydrate metabolism. However, it is currently being investigated against other diseases such as diabetes, cancer, hepatitis B, and HIV. Inhibiting alpha glucosidase temporarily delays the intestinal absorption of carbohydrates and therefore suppresses the elevation of postprandial blood glucose levels. Thus, representing a convenient method for the control of type 2 diabetes. Up till now, three approved alpha-glucosidase inhibitors are present in the market i.e., Acarbose, Miglitol and Voglibose. Therefore, research efforts seeking novel inhibitors with improved efficacy and lower side effects are increasing.
Choline esterase inhibition assay
EGP250.00 – EGP600.00 / SampleAcetyl and butyryl choline esterases (AChE and BChE), are related enzymes found across the animal kingdom. AChE role as neurotransmitter is well known several years ago, however, the role of BChE is newly emerging. Choline esterases are widely targeted for their possible therapeutic value and toxicity, inhibitors of choline esterase are used for a variety of disorders such as Alzheimer’s disease (AD) and conversely for their toxic potential as pesticides. BChE exhibits broader substrate specificity, that can act on both acetyl and butyryl choline, while AChE only acts on the acetylcholine. However, it is not acting as a neurotransmitter, recently the effect of BChE on the hydrolysis of ghrelin is reported. Ghrelin serves as a stimulant for hedonic feeding, promoting food intake and fat storage. Therefore, it can regulate feeding behavior and food intake.
Available tests
- Acetylcholine esterase inhibition
- Butyryl choline esterase inhibition
Testing protocols:
1- Initial screening at two concentration levels (100 and 10 ug/mL or uM)
2- IC50 (5 concentrations).
N.B. kinetic measurement is not available right now
COX inhibition assay
EGP900.00 – EGP4,500.00 / SampleCOX Inhibition Assay
Cyclooxygenase-2 or COX-2 is the enzyme responsible for the synthesis of prostanoids from arachidonic acid. Besides its role in inflammation, it is currently under study for a role in cancer and cardiovascular diseases.
We are offering an assay crucial for the field of drug discovery. We assay the inhibitory effect of new molecules, natural or synthesized, on both COX-1 and COX-2 enzymes.
Pancreatic lipase inhibition assay
EGP250.00 – EGP500.00 / SamplePancreatic Lipase Inhibition Assay
Pancreatic lipase is considered as a promising target for the treatment of obesity and metabolic syndromes. The digestion of lipids by pancreatic lipase is considered as main source of excessive calory intake. Therefore, it can be considered as a target for developing anti-obesity agents. Despite huge research in this area, up till now only few drugs are entered clinical trials as pancreatic lipase inhibitors. Orlistat is the only FDA approved drug as lipase inhibitor till now.
The assay is carried out using porcine pancreatic lipase and ortho-nitrophenol do-decanoate (ONPD) as substrate. Upon hydrolysis by the enzyme, ONPD turns to yellow colored ortho-nitrophenol which can be measured colorimetrically and correlated to the enzyme activity.
Tyrosinase inhibition activity
EGP250.00 – EGP600.00 / SampleTyrosinase is an enzyme that plays a crucial role in melanin production, which is responsible for the color of skin, hair and eyes. It is a copper-containing enzyme that catalyzes the rate limiting step in melanin biosynthesis, converting tyrosine to DOPA. Tyrosinase is located in melanocytes, which are specialized in producing melanin. The enzyme is involved in two reactions of melanin synthesis, the monophenol hydroxylation (conversion of tyrosine to L-Dopa) and the conversion of o-diphenol to their corresponding o-quinone (dopachrome). Inhibition of tyrosinase activity has been targeted for the improvement and prevention of hyperpigmentation.
Avaliable assays and expected output:
- Quick screening (two dose levels)
- IC50 determination (five concentrations)
N.B. Kinetic measurement is not available right now.