in-silico PCR
PCR primer design and validation
EGP0.00 / reactionPCR primers define the target region to be amplified and generally range in length from 15–30 bases. Primers shall fulfil several requirements to be valid such as GC-content of 40–60%, No three G or C residues in a row near the 3′-end of the primer and the melting temperature (Tm) of both two primers, to be within 5°C.