IFN‐γ (Interferon Gamma) ELISA Assay

It’s secreted by natural killer cells and activated T cells. It plays a significant role in activation of macrophages, intermediate antibacterial and antiviral immunity. Induces antigen presentation and orchestrate activation of the innate immune system.

Assay Principle

ELISA plates are pre-coated with an antibody specific to Rat IFN-γ. After adding samples/standards, a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each well and incubated. The optical density of IFN-γ conjugated with the biotinylated detection antibody is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat IFN-γ.