qPCR
PCR primer design and validation
EGP0.00 / reactionPCR primers define the target region to be amplified and generally range in length from 15–30 bases. Primers shall fulfil several requirements to be valid such as GC-content of 40–60%, No three G or C residues in a row near the 3′-end of the primer and the melting temperature (Tm) of both two primers, to be within 5°C.
Quantitative PCR services (qPCR)
EGP800.00 – EGP1,300.00 / sampleQuantitative gene expression analysis allows the researcher to precisely measure functional gene activity. Various questions that could be addressed via gene expression analysis include detecting alteration of gene expression due to pharmacological treatment, the oncogenic transformation of cells and other pathological conditions such as infectious diseases and autoimmune disorders.