HPLC-QTOF-HRMS

The application of mass spectrometry (MS) coupled to liquid chromatography (LC) has been recognized as a revolutionary breakthrough in the analysis and characterization of compounds. LC can efficiently separate compounds from a variety of complex samples, and MS, especially multistage (tandem), can be used to detect analytes with superior sensitivity. While DAD and fluorometric detectors can accomplish the same task with good sensitivity, insufficient selectivity and interference from molecules with similar chromatographic behavior remain the major drawback of these techniques.

The applications of LCMS in the area of natural products, as example, is incomparable to other techniques including; preliminary screening and dereplication, quality control and fingerprinting, and metabolism and pharmacokinetics studies.