Total soluble carbohydrate content

Carbohydrates are one of the most important components in foods and plants. They can be found as separate molecules or bound to other components such as lipids (glycolipids), and proteins (glycoproteins).

Anthrone method is one of the most commonly used methods for the determination of soluble carbohydrates such as monosaccharides. Sugar reacts with anthrone under acidic conditions to yield a blue-green color. The sample is mixed with sulphuric acid and heated to obtain the corresponding furfural, which in turn reacts with anthrone to get the color. The concentration of the sugars in the provided sample can be determined calorimetrically using a standard calibration curve of glucose.