Cellular Drug Uptake By Flow Cytometry

• Cellular uptake plays an essential role in drug bioactivity, for example, the internalization and accumulation of drugs into cancer cells are crucial for the therapeutic effect against tumors.
• Flow cytometry has the advantage of measuring the fluorescence of individual Cells, accordingly it can measure the cellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
• Flow cytometry can measure all aspects of cellular pharmacokinetics including the intracellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
• with relatively weak fluorescence due to high laser excitation and a high-gain photomultiplier system.
• Non-fluorescent drugs can be evaluated as well after conjugation with a suitable fluorophore.

Applications

• Assessment of the cellular pharmacokinetics of fluorescent or fluorescent labelled drugs either concentration-dependent or time-dependent.
• Cellular Pharmacokinetic studies of formulated (nanoparticles) versus non-formulated drugs.
• Cellular pharmacokinetics studies of different drugs alone and in combination with cellular entrapment enhancer (Such as P-glycoprotein inhibitors) in naïve as well as efflux pump over expressing cells (such as MCF-ADR).

Types of Tests

• Concentration dependent

• Time dependent

• Formulated / non-formulated

• With entrapment enhancer

You can also check our other services:

1- Cytotoxicity Assays

2- Flow cytometric assays

3- Multipurpose treated-flasks

4- Cell migration / wound healing assay

5- Western Blotting