Pancreatic Lipase Inhibition Assay

Pancreatic lipase is considered as a promising target for the treatment of obesity and metabolic syndromes. The digestion of lipids by pancreatic lipase is considered as main source of excessive calory intake. Therefore, it can be considered as a target for developing anti-obesity agents. Despite huge research in this area, up till now only few drugs are entered clinical trials as pancreatic lipase inhibitors. Orlistat is the only FDA approved drug as lipase inhibitor till now.

The assay is carried out using porcine pancreatic lipase and ortho-nitrophenol do-decanoate (ONPD) as substrate. Upon hydrolysis by the enzyme, ONPD turns to yellow colored ortho-nitrophenol which can be measured colorimetrically and correlated to the enzyme activity.

Testing protocols:

1-    Fast screening at two concentration levels (1mg and 100 ug/mL)

2-    IC50 (5 concentrations).

N.B. kinetic measurement is not available right now

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