PCR Primer Design and Validation  

PCR primers define the target region to be amplified and generally range in length from 15–30 bases. Primers shall fulfil several requirements to be valid such as GC-content of 40–60%, No three G or C residues in a row near the 3′-end of the primer and the melting temperature (Tm) of both two primers, to be within 5°C.

Applications

Designing primer sequences to get successful nucleic acid-based assay such as conventional PCR, RT-qPCR or Sanger sequencing & others.

Our in-silico PCR services to integrate their molecular biology expertise with computational pipelines to design your primers and use them to run an in-silico PCR to predict the success of your planned nucleic acid-based assay before taking the risk to use your precious samples in an actual PCR experiment.

What exactly do we provide?

PCR primer design and analysis.

Outputs/deliverables

• Designed forward and reverse primer sequences customized based on your requested downstream assay and primer analysis report.
• Results of in-silico PCR experiments.

You can also check our other services:

1- Molecular microbial Identification

2- ChemiDoc Imaging

3- MicroRNA extraction & quantification

4- Quantitative PCR services (qPCR)

5- Conventional PCR services

6- Western Blotting