Total soluble carbohydrate content

Carbohydrates are one of the most important components in foods and plants. They can be found as separate molecules or bound to other components such as lipids (glycolipids), and proteins (glycoproteins).

Anthrone method for determination of carbohydrates

Anthrone method is one of the most commonly used methods for the determination of soluble carbohydrates such as monosaccharides. Sugar reacts with anthrone under acidic conditions to yield a blue-green color. The sample is mixed with sulphuric acid and heated to obtain the corresponding furfural, which in turn reacts with anthrone to get the color. The concentration of the sugars in the provided sample can be determined calorimetrically using a standard calibration curve of glucose.

Results and data presentation

Concentration of total soluble carbohydrates/g extract or plant material and a standard calibration curve of glucose are delivered.

Sample form

Sample can be provided as plant powder, 80% ethanol extract or aqueous extract.

N.B. extra fees will be applied for extraction process if the provided sample is plant powder.

You can also check our other services:

1- Purification using flash chromatography

2- Supercritical fluid extraction

3- Extraction of natural products

4- Fractionation of extracts (Partitioning / Diaion)

5- Sephadex LH-20 open columns (size exclusion)

6- Total anti-oxidant capacity

7- Determination of phenolics and flavonoids

8- Choline esterase inhibition assay

9- Alpha glucosidase inhibition assay