pharmacokinetics
Cellular Drug Uptake by Flow cytometry
EGP700.00 – EGP1,000.00 / compound- Cellular uptakeplays an essential role in drug bioactivity, for example, the internalization and accumulation of drugs into cancer cells are crucial for the therapeutic effect against tumors.
- Flow cytometry has the advantage of measuring the fluorescence of individual Cells, accordingly it can measure the cellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
- Flow cytometry can measure all aspects of cellular pharmacokinetics including the intracellular uptake, entrapment, and accumulation of any fluorescent drug accurately and quantitatively.
- with relatively weak fluorescence due to high laser excitation and a high-gain photomultiplier system.
- Non-fluorescent drugs can be evaluated as well after conjugation with a suitable fluorophore.
You can also check our other services:
1- Cytotoxicity Assays
2- Flow cytometric assays
3- Multipurpose treated-flasks
4- Cell migration / wound healing assay
5- Western Blotting
Cellular Drug Uptake by HPLC
EGP1,250.00 / compoundMeasuring the transport or internalization of biologically active molecules (drugs, nanoparticles,) into cells that are key factors in
- The discovery and development of novel drugs.
- Determination of the compound’s potency and efficacy.
- Limiting the toxic effects and increasing the desired effects.
- Estimating the drug selectivity that often depends on the presence or absence of specific transporters.
You can also check our other services:
1- Cytotoxicity Assays
2- Flow cytometric assays
3- Multipurpose treated-flasks
4- Cell migration / wound healing assay
5- Western Blotting
Histopathologic examination
EGP0.00Histopathologic examination of whole-body organs
Histopathology refers to the microscopic examination of tissue by an expert pathologist in order to study the manifestations of a disease or toxicity. Following experimentation on animals, a histopathologic check can reveal a wide range of insights on different effects that took place on animal organs.
Our services include
- Organ harvest.
- Tissue fixation in paraffin
- Processing and sections preparation
- Staining
- Pathological check by experts in the field
- Interpretation and detailed report with high-quality images
Our list of checks include:
- All gross lesions • Heart •Pancreas • Stomach (forestomach, glandular stomach)
- Adrenal gland • Ileum •Jejunum •Parathyroid gland •Teeth
- Aorta •Peripheral nerves • Testis • kidney • pituitary gland • Thymus
- Brain (including sections of cerebrum, cerebellum, and medulla/pons)
- Caecum • Lacrimal gland (exorbital) • Prostate • Thyroid • Cervix
- Liver • Rectum • Tongue • Lung • Salivary gland • Trachea • Skin
- Colon • Lymph nodes (both superficial and deep) • Urinary bladder
- Seminal vesicle • Mammary gland • Skeletal muscles • uterus (including cervix)
- Upper respiratory tract, including nose, turbinates, and paranasal sinuses
- Eye (including retina) • Oesophagus • Urethra • Ovary
- Spinal cord (at three levels: cervical, mid-thoracic, and lumbar)
- Femur with joint • Olfactory bulb • Spleen • Vagina
- Sections of bone marrow and/or a fresh bone marrow aspirate
Service Pricing
Price is determined on a case-by-case basis according to the required experimental study.
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Pharmacokinetic studies in animals
EGP0.00Preclinical pharmacokinetic studies in animals
Pharmacokinetics (PK) describes everything that the body does to the drug after administration and includes absorption, distribution, metabolism, and excretion. Pharmacokinetic studies are an essential part of the drug development process. Understanding the pharmacokinetic and pharmacodynamic behavior of a given therapeutic drug is an essential element of understanding its effectiveness and safety, as well as identifying the proper dosage and distribution. PK studies can be carried for the drug molecule alone or for the whole dosage form.
Experimental designs
- Available animal models: rats & mice.
- We have the necessary expertise in the following routes of administration:
- Oral
- IP
- SC
- IV (only in rats)
Available tests
- Collecting blood samples (plasma on EDTA & citrate, as well as serum).
- Drug quantification using LC-MS
- Whole aorta extraction
- Extraction of hippocampus and frontal cortex
- Fetal double staining for bone and cartilage
- Complete semen analysis
- Urine collection and analysis
- Mammalian bone marrow chromosome aberration test
- Mammalian erythrocyte micronucleus test
- Gross necropsy examination
Service Pricing
Price is determined on a case-by-case basis according to the required experimental study.